What process involves amplifying a DNA sequence using PCR and incorporating it into a plasmid?

The process of amplifying a DNA sequence using PCR (Polymerase Chain Reaction) and then incorporating that amplified sequence into a plasmid is known as cloning. Cloning involves creating copies of a specific DNA fragment, which can then be inserted into a plasmid for further study or manipulation. This allows scientists to produce multiple copies of a gene or DNA segment for various applications, such as gene expression studies, producing proteins, or creating genetically modified organisms.

In this context, PCR is utilized to amplify the desired DNA sequence, making it suitable for inserter, and the plasmid acts as a vector to maintain and replicate the DNA in a host organism, typically bacteria, during cell division. This is a foundational technique in biotechnology and molecular biology, enabling further research and developments in medical and industrial applications.