What type of assay is ELISA?

Prepare for the Biotech Certification Test. Study with quizzes, in-depth explanations, and helpful hints. Boost your confidence and knowledge to excel in your exam journey!

ELISA, which stands for Enzyme-Linked Immunosorbent Assay, is a highly specific test primarily used for detecting and quantifying proteins, such as hormones, antibodies, or antigens in a sample. The test utilizes specific antibodies that bind to the target protein, which is then quantified using an enzyme-linked system that produces a measurable signal, typically a color change. This specificity allows for unparalleled precision when evaluating complex mixtures and determining the concentration of a particular protein of interest.

The other options do not accurately represent the function of ELISA. The method for evaporating solvents pertains more broadly to laboratory techniques that focus on concentration or isolation, not specific protein detection. Qualitative analysis for volatile compounds involves methods for determining the presence of compounds that easily vaporize, which is different in focus and approach compared to the target-specific nature of ELISA. Additionally, microbial culture tests are used to grow and identify microorganisms, which is unrelated to the protein detection capabilities of ELISA. Thus, the characterization of ELISA as a highly specific test for protein concentration is indeed the accurate choice.

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